Hif1a western blot

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August undefined, 2024

WebWestern blot analysis of HIF1A in cobalt chloride treated/untreated COS-7 nuclear extracts. Sample was incubated in HIF1A monoclonal antibody (Product # MA1-16511). Fig. 4 PTBP3 increases HIF-1alpha protein levels in colon cancer cells. a-b Western blot of HIF-1alpha expression in HCT116 and SW480 cells +- PTBP3 OE grown under normoxia or … WebNational Center for Biotechnology Information

Any one have experienced with western blotting for Hif1a?

Web11 de abr. de 2024 · MCFKÉ7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies 6LPSOH:HVWHUQ 10 µg/mL See Below.QRFNRXW9DOLGDWHG HIF-1 alpha/HIF1A is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF-1 alpha/HIF1A … WebWestern blot analysis of HIF1A in Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts using HIF1A polyclonal antibody (Product # PA5-85494) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to ... blue springs florida manatee cam

Knockdown of Hypoxia-Inducible Factor 1α (HIF-1α) Promotes

Web保存条件： HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 WebWestern blot analysis of HIF1A in Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts using HIF1A polyclonal antibody (Product # PA5-85494) using … WebHIF1A Monoclonal Antibody (mgc3), Invitrogen™-Mouse Monoclonal Antibody Compre HIF1A Monoclonal Antibody (mgc3), Invitrogen™ en ... immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. clearstone associates

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WebWestern blot analysis of extracts from various cell lines, untreated (-) or treated with either DMOG (1 mM, 6 hr; +) or cobalt chloride (100 µM, 4 hr; +) as indicated, using HIF-1α (D2U3T) Rabbit mAb (upper) and β-Actin … Web8 de dez. de 2024 · The hypoxia-inducible factors (HIF) are transcription factors that activate the adaptive hypoxic response when oxygen levels are low. The HIF transcriptional program increases oxygen delivery by inducing angiogenesis and by promoting metabolic reprograming that favors glycolysis. The two major HIFs, HIF-1 and HIF-2, mediate this … clears to install headlightsWebIn Western blotting, I detected clear lower molecular band in about 40-80kDa. On the other hand, Higher molecular band was also detected. I studied that theoretical MW of HIF-1 … blue springs ford parts department

"WebKnockdown of HIF1A was achieved by transfecting MCF7 with HIF1A specific siRNAs (Silencer® select Product # S6541, S6539). Western blot analysis was performed using Nuclear enriched extracts from the HIF1A knockdown cells (treated with CoCl2, 150 µM for 48hrs) (lane 4), non-targeting scrambled siRNA transfected cells (treated with CoCl2, … " - Hif1a western blot

Hif1a western blot

Anti-HIF-1 alpha antibody [H1alpha67] (ab1) Abcam

WebRT-PCR and Western blot were used to exa-mine expression of HIF1α and SNAPC1 RNA and proteins after interference of rt-circ-HS with siRNA, respectively. The binding of rt-circ-HS with microRNA 539 (miR-539), and miR-539 with HIF1α 3' untranslated region (3' UTR), and the effects of these interactions were investigated by dual luciferase reporter gene … Web8 de jun. de 2013 · 各位GGJJ们，本人快被HIF-1a逼疯了！. 一直想把HIF-1a蛋白给做出来，试了很多办法，都没有成功！. 试过常规裂解液提蛋白，没结果。. 从论坛上看过 …

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WebDetection of Human, Mouse, and Rat HIF-1 alpha/HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Balb-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 8 hours. PVDF membrane was probed with 1 µg/mL … WebMonoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts …

Web25 de jun. de 2024 · Transcriptional analysis was used to find the possible targets in osteoarthritis. Western blot analysis, RT-qPCR and immunofluorescence staining were used to detect the changes in gene and protein levels in the vitro experiment. The expression of HIF-1α was increased in human and mouse OA cartilage. WebDoes anyone have any advice on how to successfully extract HIF1a and HIF2a from HEK293T for measurement in a western blot. These are the materials I am using: Nuclear extraction kit Abcam ...

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Web3.Western blot检测：-80℃保存的小鼠子宫组织分别提取核蛋白与胞质蛋白，加入适量蛋白酶抑制剂；将40～50 μg蛋白质进行10%SDS-PAGE胶电泳，转移至PVDF膜后，分别进行VEGF(Santa Cruz，美国)、HIF1A(Novus，美国)、β-ACTIN(北京康为世纪生物)、LAMIN-B抗体(Santa Cruz，美国)杂交，用ECL发光试剂盒(北京全式金生物)检测。

WebWestern Blot. Detection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed … blue springs ford incWebWestern blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated … clearstone accountantsWebWestern blot analysis of HIF1A in 50 µg cobalt chloride induced COS-7 nuclear extracts. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504. FIGURE 1 Effects of oxygen concentration on growth, cell-death, gene expression and metabolism in human colonoids (A) . blue springs fl cabinsWebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α ... Western blot analysis of extracts from Hep G2 cells untreated or treated with cobalt chloride (100 µM, 4 h; +) and U-2 OS cells untreated or treated with DMOG (1 ... clearstone aggregateWeb1 de set. de 2011 · DOI: 10.1016/j.ab.2011.04.037. Abstract. Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to … clearstone buildersWebHow can I get western blot bands of hif-1a? I am trying to do western blotting of tumor samples for HIF-1a. The antibody is from neomarkers and I used a dilution of 1:1000. … clearstone brooklynWebPolyclonal Antibody for studying HIF1A. Cited in 194 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, … blue springs ford parts coupon